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Use of green fluorescent protein-expressing aspergillus fumigatus conidia to validate quantitative PCR analysis of air samples collected on filters

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<title>Use of green fluorescent protein-expressing aspergillus fumigatus conidia to validate quantitative PCR analysis of air samples collected on filters</title>
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<name type="personal" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080225889">
<namePart>McDevitt, James J.</namePart>
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<namePart>American Industrial Hygiene Association</namePart>
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<dateIssued encoding="marc">2005</dateIssued>
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<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract>This study used green fluorescent protein (GFP) - expressing Aspergillus fumigatus conidia to compare quantitative PCR enumeration with direct epifluorescent microscopic filter counts of conidia collected on filters in a test chamber. In separate experiments this study initially compared white versus fluorescent light microscopy, counting of corresponding filter halves </abstract>
<note type="statement of responsibility">James J. McDevitt... [et al.]</note>
<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080580858">
<topic>Higiene ambiental</topic>
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<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080619497">
<topic>Enfermedades respiratorias</topic>
</subject>
<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080607609">
<topic>Partículas ambientales</topic>
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<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080603137">
<topic>Procesos industriales</topic>
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<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080543969">
<topic>Filtros</topic>
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<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080574901">
<topic>Calidad del aire</topic>
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<subject authority="lcshac" xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="MAPA20080549855">
<topic>Bacterias</topic>
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<topic>Higiene industrial</topic>
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<classification authority="">872</classification>
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<title>Journal of occupational and environmental hygiene</title>
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<publisher>Cincinnati, Ohio : American Industrial Hygiene Association : American Conference of Governmental Industrial Hygienists</publisher>
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<part>
<text>Vol. 2, nº 12, December 2005 ; p. 633-640</text>
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